Table 1.
Primary antibodies used in this study
| Primary Antibodies | ||||||
|---|---|---|---|---|---|---|
| # | Antigen | Pre- treatment |
Dilution | Antibody type |
Target | Source |
| 1 | PLP | ST, E | 1:1000 | mAb; mouse | proteolipid protein myelin | Serotec, Oxford, UK |
| 2 | CD68 | ST, E | 1:100 | mAb; mouse | sialomucin microglia, macrophages | Dako, Glostrup, Denmark |
| 3 | KiM1P | ST, C | 1: 5000 | mAb; mouse | pan-microglia-macrophages | W.Brück Göttingen,Germany |
| 4 | MAG | ST, E | 1: 1000 | mAb; mouse | myelin associated glycoprotein | Abcam, Cambridge, UK |
| 5 | MOG | ST, C | 1:1000 | mAb; mouse | myelin oligodendrocyte glycoprotein | Abcam, Cambridge, UK |
| 6 | CNPase | ST, E | 1: 2000 | mAb; mouse | 2', 3'-cyclic nucleotide 3'-phosphodiesterase | Sternberger Monoclonals Inc., Lutherville, USA |
| 7 | NF 150kD | ST, E | 1:2000 | Ab; rabbit | phosphorylated neurofilament chain M | Chemicon, Temecula, CA, USA |
| 8 | GFAP | ST, E | 1: 3000 | Ab; rabbit | glial fibrillary acidic protein | Dako, Glostrup, Denmark |
Ab = polyclonal antibody; C = Citrate buffer (10mM, pH 6.0); E = EDTA (EDTA 1mM, TRIS 10mM), pH 9.0; mAb = monoclonal antibody; ST = Steamer