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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Cell Microbiol. 2015 May 29;17(11):1605–1617. doi: 10.1111/cmi.12459

Fig. 8. Knockdown of JNK suppresses FOXO-mediated responses to P. gingivalis.

Fig. 8

A. TIGK cells were transfected with siRNA to JNK or scrambled siRNA (siRNA Control). Control cells were non-transfected. Cell lysates were immunoblotted with JNK antibodies or GAPDH antibodies as a loading control.

B. Transfected TIGK cells were infected with P. gingivalis 33277 MOI 10 (Pg) for 2 h and Cat, Sod2 Prdx3, Bcl-6 and IL-1β mRNA levels were measured by qRT-PCR. Data were normalized to GAPDH mRNA and are expressed relative to non-infected (NI) siRNA controls. Results are means ± standard deviation (SD); n = 3; *P < 0.05; **P < 0.01.

C. Transfected TIGK cells were infected with P. gingivalis 33277 MOI 10 (Pg) for 2 h, or left uninfected (NI), and IL-1β levels in culture supernatants were determined by ELISA. Results are mean ± SD; n = 3; *P < 0.05; **P < 0.01.