Natural abundance 13C spectra of glutamine and aspartate phantom under the
following conditions: (a) with neither nuclear Overhauser effect (NOE) nor decoupling, (b)
with NOE only, and (c) with NOE and decoupling. Each spectrum was acquired with TR = 5 s,
NA = 104, BW = 5 kHz, data point = 2048, and processed with Lorentzian broadening (LB) =
1.0 Hz. Stochastic decoupling was performed with a unit repetition time of 500 μs,
γB2 = 200 Hz. Gln5: glutamine C5; Gln1: glutamine C1; Asp4: aspartate
C4; Asp1: aspartate C1.