Fig. 5.

Effect of lycopene-mediated induction of the Akt pathway on MnSOD expression. (A) MSCs were treated with lycopene (10 μM) for 0–120 min. The levels of phosphorylated PI3K and Akt were detected by western blot analysis. (B) MSCs were treated with lycopene for 0–48 h. Total cell lysates were subjected to SDS-PAGE and probed with an anti-MnSOD antibody. (C) MSCs were treated with an Akt inhibitor prior to lycopene treatment (48 h), after which the expression of MnSOD was detected. (D) MSCs were treated with lycopene and/or an Akt inhibitor prior to H₂O₂ treatment, and then DCF-DA was assessed by confocal microscopy.