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Altered levels of differentiation-associated genes during Vit C-induced keratinocyte differentiation. Human primary keratinocytes (KC) were cultured in high Ca2+-supplemented medium with or without Vit C, as described in materials and methods. Phage contrast images of early or terminally differentiated KC (A). Protein expression of differentiation-associated genes was determined by western immunoblot analysis (B). Scale bar=20 μm.
