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. 2015 Oct 26;6(4):e0038. doi: 10.5041/RMMJ.10223

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Copyright: © 2015 Riskin and Mond.

This is an open-access article. All its content, except where otherwise noted, is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cells were maintained in prolactin-rich medium for 4 days, before they were fixed and stained with specific anti-GLUT1 primary antibody, or stained with BODIPY-TR ceramide. GLUT1 is shown in green after staining with FITC-conjugated secondary antibody. BODIPY-TR ceramide appears in red. A, D: GLUT1 signal. B, E: BODIPY-TR ceramide signal. C, F: Superimposed image. There is little overlap of GLUT1 green signal with BODIPY-TR ceramide.