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. 2015 Oct 20;109(8):1630–1638. doi: 10.1016/j.bpj.2015.08.029

Figure 1.

Figure 1

Effect of temperature on FRET for vesicles composed of 1:1 bSM/POPC with cholesterol. The samples contained 1:1 SM/POPC with (not correcting for labeled lipids present) 38 mol % (triangle) or 28 mol % (circle) cholesterol. In all figures, the samples also contained 0.05 mol % pyrene-DPPE (open symbols) or 0.1 mol % NBD-DPPE (solid symbols) and, when acceptor was present, 2 mol % rhodamine-DOPE. In all figures, the samples contained vesicles with 500 μM total unlabeled lipid dispersed in PBS, pH 7.4. F/Fo equals the ratio of donor (pyrene-DPPE or NBD-DPPE) fluorescence in the presence of acceptor (rhodamine-DOPE) to that in its absence. FRET efficiency is 1-F/Fo. In all figures, standard deviations are shown when they are larger than the symbol size; the number of samples is given in Table 1.