Figure 5.

Calculated FRET versus donor partition coefficients in domain-containing membranes in which the acceptor partitioned strongly into Ld domains. F/Fo is shown for acceptor Kp (Lo/Ld) values of 0.1 (circles), 0.05 (squares), and 10 (plus symbols). (A and B) F/Fo is shown at (A) low acceptor concentration and/or small-Ro conditions (weak FRET), and (B) higher acceptor concentration and/or large-Ro conditions. Panel (B) approximates the behavior of the NBD-DPPE/rhodamine-DOPE FRET pair in the experiments (acceptor concentration = 0.79/Ro²). F/Fo values were calculated for a bilayer that was 50% Lo domains. F/Fo values for homogeneous membranes lacking domains (and/or acceptor Kp = 1) are shown by dashed lines.