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Kinetic parameters for the laccase-catalysed reaction using enzyme electrode employing various methods of immobilization with catechol as substrate
| Immobilization method | V max (V) | K m app. (×10−5 M) | V max. (V)/K m app. (×10−5 M) |
|---|---|---|---|
| Co-cross-linking with | |||
| Bovine serum albumin | 2.8 | 5.75 | 0.4870 |
| Lysozyme | 3.05 | 11.87 | 0.2570 |
| Gelatin | 3.5 | 16.59 | 0.2109 |
| Cross-linking | 3.25 | 30.57 | 0.1063 |
| Entrapment | 3.1 | 17.89 | 0.1733 |
| Hybrid | 2.95 | 15.95 | 0.1850 |
