Table 5.

Calibration data and analytical characteristics for the different ortho-substituted phenolic compounds using laccase biosensor employing various immobilization methods

Method of immobilization	Enzyme may have leached out before the start of analysis
	LOD (×10−5 M)	Response time min.	Recovery time min.	Linearity range (×10−5 M)	R 2	Slope
Physical adsorption
Cross-linking
Guaiacol	3	4	1	4–40	0.9910	0.0961
2-aminophenol	1	4	1	2–30	0.9891	0.191
Catechol	4	5	1.15	4–50	0.993	0.0621
2-methylphenol	13.7	5	1.20	10–60	0.9902	0.0521
2-Chlorophenol	16.2	5	1.15	25–100	0.9962	0.0397
Co-cross-linking with bovine serum albumin
Guaiacol	0.5	3	0.5	0.6–10	0.9928	0.3047
2-aminophenol	0.3	3	0.5	0.5–8	0.9950	0.3527
Catechol	0.7	3	0.5	1–10	0.9934	0.2988
2-methylphenol	1	4	1	2–16	0.9893	0.2092
2-Chlorophenol	6	4	1	6–18	0.9806	0.1523
Co-cross-linking with lysozyme
Guaiacol	1	4	1	2–10	0.9941	0.2991
2-aminophenol	0.8	4	1	1–10	0.9908	0.3092
Catechol	2	5	1.10	2–20	0.9923	0.1795
2-methylphenol	4	6	1.30	4–40	0.9958	0.1336
2-Chlorophenol	10	6	1.30	20–80	0.9891	0.0935
Co-cross-linking with gelatin
Guaiacol	1.2	5	1.30	2–20	0.9922	0.195
2-Aminophenol	0.9	5	1.30	2–10	0.9991	0.2253
Catechol	3	7	1.40	3–30	0.9902	0.1113
2-methylphenol	5	7	2	6–50	0.9962	0.1081
2-Chlorophenol	12	8	2	20–100	0.9904	0.0465
Entrapment
Guaiacol	2	5	2	2–30	0.9961	0.1643
2-Aminophenol	1.2	5	2	2–20	0.9922	0.2099
Catechol	4	7	2	4–35	0.9896	0.1061
2-Methylphenol	5	7	2	6–50	0.9907	0.0695
2-Chlorophenol	10	8	2	20–100	0.9962	0.051
Hybrid
Guaiacol	1.5	4	1	2–20	0.9890	0.2811
2-aminophenol	0.5	4	1	0.6–10	0.9915	0.3013
Catechol	3	6	1.30	3–30	0.9920	0.1241
2-methylphenol	4.5	7	1.50	6–20	0.9982	0.0923
2-Chlorophenol	6.8	7	1.50	8–40	0.9981	0.0791