Figure 3.

Effect of osmotic stress on passage time and buoyant mass. FL5.12 cells were incubated in hypo- and hyperosmolar media for 30 min before and during the measurement. Passage time and buoyant mass were relatively unaffected by osmotic stress at 250, 350, and 400 mOsm/L. (A) Boxplots of passage time scaled by the median of the control (300 mOsm/L). Each data point represents the passage time of an individual cell (n = 972–1101). (B) Percentage change in the median passage time. The median passage time from each condition is normalized by the median of the control. A single point represents one replicate and the green line indicates the mean from multiple replicates (n = 3–6 for each condition). (C) Boxplots of buoyant mass scaled by the median of the control. The data shown were measured simultaneously with passage time in (A). Each data point represents the buoyant mass of an individual cell. (D) Percentage change in the median buoyant mass. The median buoyant mass from each condition is normalized by the median of the control. A single point represents one replicate and the green line indicates the mean from multiple replicates (n = 3–6 for each condition). To see this figure in color, go online.