Figure 4.

Determining deformability from passage time by accounting for cell volume. Volume is obtained by converting single-cell buoyant mass data using the population average density. (A) Passage time versus volume from the two data sets (from Fig. 3, isoosmotic and hyperosmotic conditions) in a log-log scale is fitted to the linear models (black lines) with a fixed slope and variable intercepts corresponding to the two conditions. The deformability is determined by the ratio of passage times given the same cell volume, which is acquired from the difference between the two intercepts (green arrow). (B) Dependence of deformability (percentage change in passage time based on cell volume) on the osmolarity of the media. The data used are the same as shown in Fig. 3. To see this figure in color, go online.