Figure 5.

Deformability versus density and volume for various conditions: osmotic challenge (250, 350, 400, and 500 mOsm/L), latrunculin B (LatB), staurosporine (STS), 1 μg/mL and 10 μg/mL of cycloheximide (CHX), rapamycin (Rap), and Torin 1 (Tor). Changes in deformability, density, and volume after treatments are quantified based on the isoosmotic control (untreated, 300 mOsm/L) in each experiment. Plots are divided into four quadrants, defined by two gray dotted lines. (A) The percentage change in passage time accounting for volume is plotted versus the change in density. The correlation between changes in deformability and density depends on the mechanism associated with each treatment. (B) The percentage change in passage time accounting for volume is plotted versus the change in volume. Rap, Tor, and CHX (1 μg/mL) are located in different quadrants compared with (A) (arrows). Vertical error bars represent the standard deviation of the mean. Horizontal error bars (density and volume) represent the standard error of the mean. All treatments induce a significant change in density (p < 0.0001, Wilcoxon rank sum). All treatments, except Torin 1 (p = 0.0501), induce a significant change in passage time (p < 0.0001, linear model). The data for the osmotic challenge are the same as those shown in Fig. 4. For the other conditions, we measured ∼200 cells and ∼1000 cells for density and deformability, respectively. To see this figure in color, go online.