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. 2015 Sep 11;1(8):e1500301. doi: 10.1126/sciadv.1500301

Fig. 1. Ankyrin-G/βII-spectrin microdomains sample the entire lateral membrane through microtubule-dependent localized movement.

Fig. 1

(A) Schematic depicting the en face visualization of the columnar epithelial cell lateral membrane. Representative deconvolved en face (right) image from MDCK cells stained with antibodies against endogenous ankyrin-G. Scale bar, 2.5 μm. Region of interest depicts size of area analyzed in (B). (B) Representative deconvolved en face confocal images of live MDCK cells transfected with ankyrin-G–GFP shown at time 0 (green) or 5 min (red) for control cells (top) or cells treated for 3 hours with nocodazole (Nocod.) (100 ng/ml) (second row) or 1 μM latrunculin A (LatA) (third row). Cells fixed with paraformaldehyde (bottom) were used as an imaging control. Summed coverage at 0 and 5 min shown on the right. Scale bar, 1 μm. (C) Quantification of percent lateral membrane coverage from cells in (B). One-way analysis of variance (ANOVA) followed by Tukey post hoc test; *P < 0.05 compared to control, n = 4 to 9 cells per condition.