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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cancer Res. 2015 Sep 1;75(17):3505–3518. doi: 10.1158/0008-5472.CAN-15-0139

Figure 1.

Figure 1

Cetux-CAR+ and Nimo-CAR+ T cells are phenotypically similar. (A) Numeric expansion of Cetux-CAR+ and Nimo-CAR+ T cells was determined by calculating fold expansion of CD3+CAR+ T cells, determined by flow cytometry, during each stimulation cycle. Data represented as mean ± SD, n=5. Statistical analysis by two-tailed student’s t-test. (B) Expression of CAR in CD3+ T cells was determined 24 hours after electroporation of CAR and after 28 days of expansion by flow cytometry for the IgG portion of CAR. Data represented as mean, n=7. Statistical analysis performed by two-tailed student’s t-test. (C) Median fluorescence intensity (MFI of CAR expression was determined by flow cytometry for the IgG portion of CAR after 28 days of expansion. Data represented as mean ± SD, n=7. Statistical analysis performed by two-tailed student’s t-test. (D) Proportion of CD4 and CD8 T cells in total T-cell population after 28 days of expansion measured by flow cytometry on gated CD3+CAR+ cells. Data represented as mean ± SD, n=7. (E, F) Expression of T-cell memory and differentiation markers after 28 days of T-cell expansion measured by flow cytometry in gated CD4+ and CD8+ T-cell populations. Data represented as mean ± SD, n=4. Statistical analysis performed by two-way ANOVA with Sidak’s post-test correction.