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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cancer Res. 2015 Sep 1;75(17):3505–3518. doi: 10.1158/0008-5472.CAN-15-0139

Figure 3.

Figure 3

Nimo-CAR+ T cells have impaired response to low density of EGFR. (A) Representative histograms of expression of tEGFR on EL4 cells relative to cell lines negative for EGFR. Density of EGFR expression was determined by quantitative flow cytometry. (B) Production of IFN-γ by gated CD8+ CAR+ T cells after co-culture with CD19+, tEGFR+, or CAR-L+ EL4 cells measured by intracellular staining and flow cytometry. Data represented as mean percent ± SD, n=4 and mean fluorescence intensity (MFI) ± SD, ** p<0.01 two-way ANOVA (Sidak’s post-test) and mean fluorescence intensity ± SD, n=2. (C) Phosphorylation of p38 and Erk1/2 by phosflow cytometry in gated CD8+ CAR+ T cells 30 minutes after co-culture with CD19+, tEGFR+, or CAR-L+ EL4 cells. Data represented as mean ± SD, n=2, * p<0.05, two-way ANOVA (Sidak’s post-test). (D) Specific lysis of CD19+, tEGFR+ and CAR-L+ EL4 cells measured by standard 4-hour chromium release assay. Data represented as mean ± SD, n=4, **** p<0.0001, two-way ANOVA (Tukey’s post-test).