Table 2.
Kinetic parameters of PikDH2 mutants.
| Mutants | KM (mM) | kcat (min−1) | kcat/KM (min−1M−1) |
|---|---|---|---|
| Wild | 5.7 ± 1.4 | 1.28 ± 0.19 | 225 ± 88 |
| F3746L | > 40 | N/A | 5.2 ± 0.3 |
| F3750Y | 4.1 ± 1.4 | 0.086 ± 0.013 | 21 ± 9 |
| F3750L | 7.1 ± 2.6 | 0.046 ± 0.011 | 6.5 ± 3.9 |
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