Fig. 3.
Synthetic peptides corresponding to sequenced peptides bind to HLA-A2.1. Synthetic peptides are referenced by the numbers in Table 1. JY cells were incubated with the M157–68 peptide of influenza A (8 µg/ml), in the presence or absence of one of the synthetic peptides (200 µg/ml) as a competitor, in a total volume of 250 µl of RPMI 1640 containing 5% new-born calf serum and 50 µCi of51 Cr for 3 hours. Washed target cells (2 × 103) were incubated in 200 µl with either an M1 peptide–specific, HLA-A2.1–restricted CTL, done 5.2–11 (31) (filled bars) or an HEA-A2.1–specific human alloreactive CTL line (stippled bars) at an effector to target ratio of 1.25:1. Data are the means of duplicate samples with an SD of less than 5% and are representative of at least three independent experiments.