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. Author manuscript; available in PMC: 2015 Oct 28.
Published in final edited form as: J Immunol. 2007 Sep 1;179(5):2669–2671.

Table 1.

Partial listing of data for peptides extracted from HLA-A2.1. HLA-A2.1 was purified and acid extracts prepared from the cell lines JY, C1R-A2.1, and C1R, as outlined in Fig. 1. Aliquots corresponding to 108 cells were analyzed by microcapillary HPLC–electrospray ionization–mass spectrometry. Signals represent measured ion current. Synthetic peptides corresponding to sequences S and 14, doped at 1 pmol into samples, gave signals of 60 × 105 to 70 × 105, whereas background noise gave a signal of 1 × 105. Sequences were deduced as in Fig. 2.

Peptide (M+H)+
(m/z)		 JY C1R-A2.1 C1R Sequence* Database
match
Signal
(×105)		 Yield
(fmol)		 Signal
(×105)		 Yield
(fmol)		 Signal
(×105)
1 786 9.1 137 2.0 30 -† SXPSGGXGV
2 868 2.7 41 7.9 119 -
3 871 2.9 44 6.1 98 -
4 898 11.9 179 15.8 237 - XXDVPTAAV LLDVPTAAV
5 903 4.7 71 11.8 177 -
6 930 4.9 74 - - - GXVPFXVSV
7 945 11.8 177 3.1 47 -
8 954 11.9 179 2.5 38 - SXXPAXVEX SLLPAIVEL
9 971 8.5 128 4.1 66 -
10 994 6.2 93 - - -
11 999‡ 3.5 53 - - - SXXVRAXEV
12 1018‡ 4.3 65 2.5 38 -
13 1037‡ 10.0 150 7.3 110 - KXNEPVXXX
14 1038‡ 39.4 591 8.1 122 - YXXPAXVHX YLLPAIVHI
15 1064 8.1 122 3.5 53 -
16 1095 5.2 78 - - -
17 1098 2.3 35 - - -
18 1115 11.4 171 2.9 44 -
19 1121 6.4 320§ 2.0 100§ - TLWVDPYEV TLWVDPYEV

Single letter abbreviations for the amino acid residues are: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.

*

Leu and Ile are not distinguishable by tandem mass spectrometry, and are designated X.

†

Signal was at or below background.

‡

Observed as a double charged, (M+H)2+ ion at half of this m/z value.

§

Signal obtained from 1 pmol of this synthetic standard added into sample was 2 × 106.