Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cell Microbiol. 2015 Apr 28;17(9):1376–1390. doi: 10.1111/cmi.12442

Figure 2. SLO pore formation is necessary, but not sufficient to activate PARP.

Figure 2

HeLa cells were untreated (Un) or treated with the various concentrations of purified SLO that are shown on the top of Panels A and B. The presence of PAR analyzed by Western blotting as shown, following 5 (A) or 1 (B) hours post-treatment. Formation of PAR was also assessed in HeLa cells infected with S. pyogenes strains expressing monomer or pre-pore locked mutant SLO proteins with NADase+ (Mono or Pre) or NADase− (MonoSPN™ or PreSPN™) SPN and compared with uninfected (uninfect.) cells and cells infected with strains expressing wild-type SLO, but lacking SPN (ΔSPN) or expressing NADase− SPN (NADase−). Formation of PAR was assessed by Western blotting at 5 hours post-infection, as shown (C). Migration of molecular weight standards are shown on the left. Images are representative of at least three independent experiments.