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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Nature. 2015 Sep 2;525(7568):274–277. doi: 10.1038/nature14882

Extended Figure 2. Schematic diagram showing assembly of dumbbells.

Extended Figure 2

PICs were attached to one bead via biotin-avidin linkages (yellow). To form dumbbell tethers, the other end of a small fraction of the PICs (4%) had digoxigenin linkages that could be tethered to anti-digoxigenin-coated beads (black and brown) via a 2.7 kb DNA handle. PICs not involved in tether formation served to increase the local concentration of PIC components.