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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Nature. 2015 Sep 2;525(7568):274–277. doi: 10.1038/nature14882

Extended Figure 5. Exonuclease III footprinting assay of the PIC on SNR20* long.

Extended Figure 5

In the absence of nucleotides in vitro, PIC complexes bound to the SNR20* long promoter produced barriers to exonuclease III digestion located ∼50 bp downstream of the TATA box (about −40 nt from the TSS, black arrows). These barriers depended on the presence of TFIIH and also TFIIE, which interacts with TFIIH. Upon the addition of dATP, the barriers disappeared, and the bands at pause positions were intensified between positions −30 and +30 (∼60–120 bp downstream of the TATA box, bracket).