Skip to main content
. Author manuscript; available in PMC: 2016 Oct 22.
Published in final edited form as: Cell. 2015 Oct 8;163(3):607–619. doi: 10.1016/j.cell.2015.09.027

Figure 1. Tse6 Causes Stasis from the Cytoplasm of P. aeruginosa.

Figure 1

(A) Genomic context of vgrG1, tsi6, tse6, and eagT6 in P. aeruginosa PAO1. Locus tag numbers are provided below each gene. The color of each gene corresponds to the color of its encoded protein shown in subsequent figures.

(B) Domain organization of P. aeruginosa Tse6. The boundaries for the PAAR (residues 64–175) and toxin (residues 282–430) domains are indicated. Predicted transmembrane domains are shown as dark gray rectangles.

(C) Intoxication of P. aeruginosa by Tse6 severely reduces growth. Data were derived from single-cell analysis of a parental strain (ΔretS ΔsspB pPSV38::sspB) and a derivative depleted of Tsi6 (ΔretS ΔsspB tsi6-D4 pPSV38::sspB). Bin size is 20 min and is normalized to total cells (parental, n = 15,042; tsi6–D4, n = 5,568).

(D) Tsi6 depletion strains undergo Tse6-based toxicity independent of inter-cellular toxin delivery by a functional H1-T6SS. Patches of the indicated P. aeruginosa strains grown for 24 hr at 37°C under Tsi6 depletion-inducing (+IPTG) or non-inducing (−IPTG) conditions are shown. The parental strain is the same as in (C).

See also Movies S1 and S2 and Tables S2 and S3.