FIGURE 10.
FR3-CDR3-FR4 sequences of the Ig VHDJH-Cμ, Cγ1, -Cγ2, -Cγ3, -Cγ4, -Cα1, -Cα2, and -Cε productive transcripts from CL-01 cells cultured with CD40L-293 cells, IL-4, and IL-10 for 5 days. The top sequence of each cluster is used as the term of comparison. Dashes indicate identities. The solid line above the first cluster depicts CDR3. The full dot indicates the beginning of the CH1 exon (residue −1). The sequences of the sense FR3 and antisense Cμ and Cε primers are underlined. The antisense Cγ1, Cγ2, Cγ3, Cγ4, Cα1, and Cα2 primers encompassed the sequences spanning nucleotides −285 to −308, −283 to −308, −282 to −308, −283 to −306, −772 to −796, and −758 to −783, respectively, and are not shown.