Table 1.

SINV/EEEV antigen reactivity in the EEEV MAC ELISA following inactivation by BPL

A. BPL treatment (small-scale)
Antigen Dilution	VSAA	NBR	VSAA	NBR	VSAA	NBR	VSAA	NBR	VSAA	NBR	VSAA	NBR
	0.1% BPL		0.15% BPL		0.2% BPL		0.25% BPL		0.3% BPL		Mock-treated
1:2	1.995	0.156	2.151	0.478	1.960	0.578	1.640	0.534	1.159	0.450 **	0.215	0.029
1:4	1.192	0.075	1.357	0.103	1.157	0.099	0.999	0.072	0.713	0.083	0.208	0.025
1:8	0.812	0.045*	0.885	0.055	0.724	0.045	0.593	0.039	0.437	0.039	0.118	0.022
1:16	0.537	0.038	0.537	0.041	0.416	0.038	0.346	0.031	0.277	0.032	0.063	0.021
1:32	0.428	0.030	0.315	0.030	0.274	0.029	0.197	0.030	0.204	0.025	0.027	0.024
1:64	0.219	0.036	0.196	0.026	0.155	0.024	0.123	0.024	0.129	0.025	0.022	0.022
	0.1% BPL - 50X		0.15% BPL - 50X		0.2% BPL - 50X		0.25% BPL - 50X
1:10	2.271	0.189	1.155	0.920	1.081	2.034	1.034	2.617
1:20	2.563	0.175	1.793	0.575	1.802	1.103	2.030	1.441
1:40	2.165	0.104	2.534	0.346	2.573	0.730	2.322	0.804
1:80	1.705	0.088	2.298	0.246	2.211	0.391	1.819	0.473
1:160	1.263	0.070	1.787	0.164	1.627	0.243	1.140	0.238
1:320	0.803	0.048 †	1.200	0.089	0.953	0.108	0.658	0.127

B. BPL treatment (large-scale)
Antigen Dilution	VSAA	NBR	VSAA	NBR
	0.2% BPL		0.2% BPL
	Unconcentrated		12X Concentrated
1:10	0.547	0.064	2.009	0.487
1:20	0.269	0.051	1.117	0.245 †
1:40	0.160	0.036	0.633	0.150
1:80	0.103	0.030	0.388	0.093
1:160	0.066	0.031	0.247	0.068
1:320	0.045	0.031	0.167	0.052

Abbreviations: VSAA, virus-specific antigen activity; NBR, nonspecific background reactivity.

^*OD readings in bold indicate the lowest dilution of antigen that yielded acceptable MAC ELISA results and was completely inactivated.

^**ODs in bold underlined italics indicate the dilution of antigen that yielded acceptable VSAA, but unacceptable NBR.

^†OD readings in bold italics indicate the dilution of antigen that yielded acceptable VSAA and NBR, but which was still infectious.