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. 2015 Oct 20;109(8):1619–1629. doi: 10.1016/j.bpj.2015.08.033

Figure 2.

Figure 2

Membrane interaction of GDP- and GppNHp-loaded full-length and GppNHp-bound truncated Arl3. (A) SPR sensorgrams of the binding of Arl3 (c = 2 μM) to anionic model raft membranes composed of DOPC/DOPG/DPPC/DPPG/Chol 20:5:45:5:25 (mol %). (B) Corresponding SPR data for the initial association process. The error bars represent the standard deviation from three to six measurements. (C) Surface pressure/time isotherms for the membrane interaction of Arl3 with anionic lipid raft monolayers. (D) Corresponding time-dependent IRRA spectra for the amide-I′ region of membrane-bound Arl3•GppNHp. All IRRA spectra were recorded with p-polarized light at a 35° angle of incidence because the use of p-polarized light resulted in larger signals and better signal/noise ratios (cf. Fig. S2). To see this figure in color, go online.