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. 2015 Oct 20;109(8):1619–1629. doi: 10.1016/j.bpj.2015.08.033

Figure 4.

Figure 4

Membrane partitioning of full-length and truncated Arl2/3 in the different nucleotide-bound states. All AFM images showed a defect-free, continuous lipid bilayer on mica with isolated liquid-disordered (ld) domains in a liquid-ordered (lo) membrane matrix at room temperature before injection of proteins (cf. Fig. S3). Representative AFM images after the addition of Arl2/3•GDP, Arl2/3•GppNHp, and ΔArl2/3•GppNHp to a membrane consisting of DOPC/DOPG/DPPC/DPPG/Chol 20:5:45:5:25 (mol %) are shown. The overall height of the vertical color scale corresponds to 6 nm for Arl3•GDP, ΔArl3•GppNHp, and ΔArl2•GppNHp, and 12 nm for Arl3•GppNHp, Arl2•GppNHp, and Arl2•GDP. The scale bar represents 1 μm for all images. To see this figure in color, go online.