Figure 5.

Translocation time per nucleotide versus temperature for αHL (solid circles) and AL pores (open circles) measured at a voltage of 100 mV. The plain curves are fits to the model presented in the text. They could equally be fitted to Arrhenius law, leading to an enthalpic barrier of 35k_BT for both pores. At high temperature, the value of the translocation time in AL deviates significantly from the fit. This is due to the fact that at this short timescale, our detection system reaches its limits. This effect has already been reported for protein transport through αHL and AL (9). Here, the αHL data set is not affected by this because of its much higher signal/noise ratio.