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. Author manuscript; available in PMC: 2015 Oct 28.
Published in final edited form as: FEBS Lett. 2013 Jul 12;587(17):2753–2766. doi: 10.1016/j.febslet.2013.07.012

Figure 1. Comparison of three methods of fitting data to the Michaelis-Menten equation.

Figure 1

A. Data fit by nonlinear regression to a hyperbola. B. Data fit to a Lineweaver-Burk reciprocal plot. The gray line shows the fit obtained after omitting the point at the lowest substrate concentration. C. Data fit using the Eadie-Hofstee equation. In each figure, the equation and the resulting kcat and Km values are displayed.