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. Author manuscript; available in PMC: 2016 Jan 30.
Published in final edited form as: J Mol Biol. 2014 Oct 13;427(2):443–453. doi: 10.1016/j.jmb.2014.10.003

Figure 5.

Figure 5

The 4Trp variant. a. Refolding into 0.6 M urea was monitored by average fluorescence lifetime. Two kinetic phases, similar to wild type, are observed. The extrapolated signal of the unfolded state (U) and the signal of the final equilibrium state (N) at this condition are indicated. b. Equilibrium urea denaturation monitored by average fluorescence lifetime. Data are fit with a two-state model.