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. Author manuscript; available in PMC: 2015 Oct 28.
Published in final edited form as: Aging Cell. 2010 Mar 6;9(3):358–371. doi: 10.1111/j.1474-9726.2010.00562.x

Fig. 4. The recruitment kinetics of GFP tagged WRN, BLM and RECQL4 proteins at the site of laser induced DNA damage.

Fig. 4

The HeLa cells were transfected with GFP tagged WRN, BLM or RECQL4 plasmids. After 24 hr post transfections, the cells were laser microirradiated within the nucleus with 435 nm laser light at 21% laser intensity. The images were captured for 60 seconds through FRAP channel using confocal microscope. The association of GFP-tagged WRN, BLM and RECQL4 proteins is shown at different time intervals after microirradiation in panel A, B, and C, respectively. Arrow represents the site of laser irradiation. The normalized intensity values of the kinetic of association of different proteins have been represented in panel D.