TABLE 1.
Effect of spectinomycin and neomycin on the rate of spontaneous reverse translocation
| Antibiotic | kobs1 (min−1) | A 1 a | kobs2 (min−1) | A 2 a |
|---|---|---|---|---|
| Water | 0.15 ± 0.01 | 0.98 ± 0.01 | n/a | n/a |
| Neomycin | 0.39 ± 0.02 | 0.99 ± 0.01 | n/a | n/a |
| Spectinomycin | 0.05 ± 0.01 | 0.29 ± 0.02 | 0.54 ± 0.02 | 0.26 ± 0.02 |
| Spc + Neo | 0.07 ± 0.02 | 0.33 ± 0.03 | 0.72 ± 0.01 | 0.43 ± 0.02 |
| Preincubated with spectinomycin | ||||
| Spectinomycin | 0.04 ± 0.01 | 0.54 ± 0.06 | 0.30 ± 0.03 | 0.13 ± 0.01 |
| Spc + Neo | 0.06 ± 0.01 | 0.56 ± 0.01 | 0.44 ± 0.02 | 0.06 ± 0.05 |
a
A1 and A2 are amplitudes of the reactions (Methods, Supporting Information).