TABLE 2.
Diffraction and refinement statistics for 70S ribosome in complexes with spectinomycin and neomycina
| Dataset | + Spectinomycin | + Spectinomycin + Neomycin |
|---|---|---|
| Resolution (Å) | 183–3.93 (183–4.2)b | 182–3.5 (182–3.85)b |
| Completeness (%) | 75.8 (89.4)b | 72.1 (91.3)b |
| No. of reflections | 390,966 | 521,699 |
| No. of crystals | 7 | 6 |
| Redundancy | 3.4 | 3.3 |
| I/σ | 8.4 | 8.6 |
| Overall χ2 | 1.4 | 1.4 |
| Rmerge (%) | 10.9 | 10.4 |
| Refinement | ||
| Resolution (Å) | 70–3.93 | 70–3.5 |
| No. of reflections | 390,759 | 521,145 |
| Rfree Set | 19,244 | 25,274 |
| No. of atoms | 284,067 | 284,235 |
| R/Rfree (%) | 25.8/31.1 | 26.4/30.6 |
a
Space group P212121. Unit cell a = 208.9 Å, b = 379.2 Å, c = 739.3 Å, α = β = γ = 90°.
b
Values in parenthesis are for the resolution range with a high level of data completion.