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. Author manuscript; available in PMC: 2015 Oct 28.
Published in final edited form as: Langmuir. 2015 Sep 8;31(37):10310–10317. doi: 10.1021/acs.langmuir.5b02416

Figure 5.

Figure 5

A dual-lane flowcell for imaging two DNA substrates in the presence of buffer flow. (A) Cartoon schematic of the Y-shaped flowcell with two inlets and one outlet port. Each lane is 9 mm wide and separated by a 2 mm tape spacer (gray). Bottom panel: an image of yellow and blue food dye loaded into each of the two lanes. Scale bar: 5 mm. The lanes remain fluidically isolated for over 1 h. (B) Images captured from each lane during a single experiment. Lane 1 was assembled with λ-DNA, while lane 2 contained nucleosomecoated λ-DNA. Both channels were labeled with YOYO-1 DNA intercalating dye (Life Technologies). Nucleosomes were tagged with anti-FLAG QDs (magenta; 705 nm) and were exclusively observed in the right channel. Scale bar: 4 µm.