Table 1.
PCR primers and conditions used in sequencing feline GUSB gene
| Exon | Sequence (5′‐ 3′) | Tm (°C) | Extension (Second) | Amplicon (bp) |
|---|---|---|---|---|
| 1 | Forward: CATCTACCTGTATTTCTGCGGATACAA | 60 | 30 | 507 |
| Reverse: CCGTTTCCTCCCTCGCAGTC | ||||
| 2 | Forward: TTCTTCCTGCAGGCCAGGTG | 60 | 30 | 386 |
| Reverse: TGGGAACCACACACCCCAAG | ||||
| 3 | Forward: CGCCCTGACTTGCTGTTCCT | 60 | 30 | 322 |
| Reverse: CCAGGGCCTCACGTCTGCTA | ||||
| 4 | Forward: GCAGGGTGGCCCTAGCAGAC | 62 | 30 | 421 |
| Reverse: GCCCGTGCCAGTTCAGACAG | ||||
| 5 | Forward: CACTGCAGGTTGGGCTCCAG | 62 | 30 | 442 |
| Reverse: GCCTCCCTACCAAGCGGTGA | ||||
| 6–8 | Forward: GCCAGCACGGATGCCTCACA | 62 | 45 | 949 |
| Reverse: GCCTGCCCCAGAGCCAACCT | ||||
| 9 | Forward: TCGGGAGGGAGGAAGGGTTT | 60 | 30 | 302 |
| Reverse: CTCCTCCCAGCAGCCTGTGTT | ||||
| 10 | Forward: TTTTCTGCAGCCCCCTGTTG | 60 | 30 | 368 |
| Reverse: GACGGGTGGGAGAGGAGCAC | ||||
| 11 | Forward: TGGTCACGTCACATGGATCT | 60 | 30 | 366 |
| Reverse: TAGAGCTTTCCCCCTCGTTT | ||||
| 12a | Forward: CCCGTTTGGCCCATCAGAAG | 60 | 45 | 1,016 |
| Reverse: GGAGCAAGACAGGTGGCATCA | ||||
| 12b | Forward: TCCTTTTGGATGATGCCACCTG | 60 | 45 | 1,090 |
| Reverse: GCTCCCCCTGAATGCTCCAC |
Sources: NCBI‐Genbank (Gene ID: 493879, http://www.ncbi.nlm.nih.gov, Ensembl (Felis_catus_6.2, ENSFCAG00000006928), http://www.ensembl.org and UCSC Genome Bioinformatics (ICGSC Felis_catus_6.2/felCat5), http://genome.ucsc.edu.