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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Dig Liver Dis. 2015 Jul 26;47(11):964–972. doi: 10.1016/j.dld.2015.07.012

Figure 2.

Figure 2

[A] Cross-section of a normal extrahepatic bile duct with cholangiocytes polarized around the bile duct lumen. [B] A higher magnification image of the cross-section shows intact tight junction (blue arrows) characteristic of biliary epithelia. [C] A representative picture shows isolated extrahepatic cholangiocytes cultured on transwells. [D] A higher magnification image of cultured cholangiocytes reveals intact tight junctions (blue arrows) and an abundance of microvilli (red arrow) at the apical surface. [E] Cultured cholangiocytes have vesicular invaginations at the apical and basolateral poles, suggestive of active secretory and absorptive processes characteristic of bile duct epithelia.