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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Nat Neurosci. 2015 Sep 21;18(11):1606–1616. doi: 10.1038/nn.4116

Figure 5.

Figure 5

5HT1ARs in DG GCs are necessary and sufficient for the neurogenic effects of fluoxetine. a) Mice used for panels b–d were randomly chosen from behavioral cohort in Figure 1. n=8 per group. b) Proliferation results. The number of BrdU-positive cells was analyzed by Two-Way ANOVA (F(1,28)=10.66, p=.0029). *** indicates p<.0001 for Control Vehicle vs Fluoxetine and POMC-Cre/fl1A Vehicle vs Fluoxetine and p=.0007 for Control Fluoxetine vs POMC-Cre/fl1A Fluoxetine (Tukey’s). c) The number of young abGCs. The number of Dcx-positive cells was analyzed by Two-Way ANOVA (F(1,28)=5.292, p=.0291). *** indicates p<.0001, and * indicates p=.0247 for Control Fluoxetine vs POMC-Cre/fl1A Fluoxetine (Tukey’s). d) The number of young abGCs with tertiary dendrites. The number of Dcx-positive cells with tertiary dendrites was analyzed by Two-Way ANOVA (F(1,28)=4.954, p=.0343). *** indicates p<.0001, ** indicates p=.0015, and * indicates p=.0212 (Tukey’s). e) Mice used for panels f–h were randomly chosen from behavioral cohort in Figure 3. n=8 per group. f) Proliferation results. *** indicates p<.0001 (Two-Way ANOVA; treatment effect only) g) The number of young abGCs. *** indicates p<.0001 (Two-Way ANOVA; treatment effect only). h) The number of young abGCs with tertiary dendrites. *** indicates p<.0001 (Two-Way ANOVA; treatment effect only). i) Mice used for panels f–h were randomly chosen from behavioral cohort in Figure 4. n=8 per group. j) Proliferation results. The number of BrdU-positive cells was analyzed by Two-Way ANOVA (F(1,28)=23.7, p<.0001). *** indicates p<.0001 (Tukey’s). k) The number of young abGCs. The number of Dcx-positive cells was analyzed by Two-Way ANOVA (F(1,28)=6.311, p=.0180). ** indicates p=.0014 (Tukey’s). l) The number of young abGCs with tertiary dendrites. The number of Dcx-positive cells with tertiary dendrites was analyzed by Two-Way ANOVA (F(1,28)=8.031, p=.0084). ** indicates p=.0075 (Tukey’s). Mean lines and error bars throughout the figure represent mean ± SEM. V: Vehicle. F: Fluoxetine. F(8): Fluoxetine (8 wks). F(11): Fluoxetine (11 wks).