Ethanol inhibits ligand-driven but not N1IC-driven CBF-1 promoter activity in HCASMC. CBF-1 promoter activity was determined by luciferase assay in SMC treated with or without recombinant Notch ligand Delta-like ligand 4 (DLL4, 2 μg/ml), or transfected with constitutively active Notch 1 IC (N1IC), in the absence or presence of EtOH (25 mM, 24h). *p<0.05 vs basal, # p<0.05 vs DLL4 alone. Data are mean± SEM (n=3).