Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2015 Oct 7;39(11):2115–2122. doi: 10.1111/acer.12875

Figure 5.

Figure 5

Ethanol inhibits SMC γ-secretase activity. (a) γ-secretase activity in solubilized HCASMC membranes was measured using a fluorogenic peptide substrate (C-terminal fragment of β-APP) as described in Methods. DAPT was used as a control γ-secretase inhibitor (GSI). Data are mean ±SEM, n=3. *p<0.05 vs control, # p<0.05 vs DLL4 alone. (b) HCASMC membranes from cells treated with or without EtOH were incubated with flag-tagged Notch based substrate (N100Flag). Representative Western blots show detection of N100Flagsubstrate and its cleavage product under control and EtOH conditions. Blots were reprobed with GAPDH as a loading control.