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The γ-secretase inhibitor, DAPT, and Ethanol alone inhibit Notch signaling and proliferation in HCASMC to a similar extent. (a) Notch target gene HRT2 mRNA levels, determined by RT-PCR, in HCASMC treated (24 h) with or without or DAPT (20 μM) or EtOH (25 mM) (n=3, * p<0.05 vs control). (b) Representative Western blots for proliferating cell nuclear antigen (PCNA) in HCASMC treated +/− DAPT or EtOH. Blots reprobed with GAPDH as a loading control.
