Expression of anti-apoptotic and pro-apoptotic effectors/regulators during retinal development. Western blot assay demonstrated that the activation of p-Bcl2 (A) decreases with age. The expression of NF-κB (B) is increased in the early developmental stages in tubby retinas (P4 and P7), but decreases thereafter. Densitometric analysis of the bands is shown beneath the corresponding western figure. (C) qRT-PCR analysis showed that the level of Chop mRNA in tubby is higher than the wt from P4 through P28. Data shown are mean ± SEM, N=8–10. *P<0.05, **P<0.005 (compared between tubby and age-matched wt using the unpaired student t-test), #P<0.05 (compared among tubby at different ages using one way ANOVA for A and C, or compared between each two groups using the unpaired student t-test for B).