Fig. 1.

8-OH-DPAT prevents RPE cell killing by mitochondrial oxidative stress. ARPE19 cells were seeded at 70% confluence in medium containing with 1% FBS and allowed to grow overnight. Then cells were treated with 300 μM paraquat and increasing amounts of 8-OH-DPAT, as indicated. Two days later, cells were stained with CellTiter Aqueous (Promega) and the absorbance read in a plate reader. Survival of cells was calculated using the absorbance of cells not treated with paraquat as 100%.