Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Exp Cell Res. 2015 Jul 27;338(2):170–182. doi: 10.1016/j.yexcr.2015.07.025

Figure 6.

Figure 6

Redistribution of myosin V and VI following Ad5 infection. Confocal micrographs of HeLa cells infected with Ad5 wt (1000 VP/cell) imaged at 21 hpi. (A) Double staining was performed using anti-MV antibody (red) and anti-72 kD antibody (green) and counter-stained with DAPI (blue). Redistribution of nuclear myosin V to the Type B viral DNA centers is indicated by arrows. (B) Superresolution SIM reconstruction images of MV (red), 72 kD (green), and DAPI (blue) demarcate the close association between MV and the replication factory periphery. (C) Double staining was performed using anti-MVI antibody (red) and anti-72 kD antibody (green) and counter-stained with DAPI (blue). Redistribution of nuclear myosin VI adjacent to type A dots is indicated by arrowhead and localization inside Type B replication centers is marked by arrows. (D) Superresolution SIM reconstruction images of MVI (red), 72 kD (green), and DAPI (blue) show the distribution of MVI within the replication factory.