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. 2015 Oct 27;48:59. doi: 10.1186/s40659-015-0051-6

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — HLA Class II Expression in DCs derived from CIITA targeted hESCs. a RT-PCR analysis of CD83, CD86, CD11c, DRA, DPA, DQA, Ii, CIITA, HLA-E and β2M in DCs derived from CIITA-targeted hESCs. All groups were compared with CIITA ^+/+ hESCs group. b FACS analysis of HLA II expression in DCs, which were defined by the co-expression of CD83 and CD86. And compare the percentage of HLA II⁺. Significance was assessed by a t test. The data are expressed as the mean ± SEM, n ≥3. ***P < 0.001, ** P < 0.01, * P < 0.05