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. 2015 Oct 27;15:811. doi: 10.1186/s12885-015-1794-8

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Overexpression or knockdown of Piwil1 in human endometrial cancer cell lines. a Stable transfection of Ishikawa cells with shRNA against Piwil1 and HEC-1B cells with Piwil1 expression plasmids. The percentage of transfected cells with fluorescence was > 95 %. (b and c) RT-qPCR and western blot demonstrated expression level of Piwil1 in Ishikawa, Ishikawa^NT and Ishikawa^shPiwil1 cells or HEC-1B, HEC-1B^EV and HEC-1B^exPiwil1 cells (*P < 0.05). d Representative immunofluorescence images showing Piwil1 expression in Ishikawa^NT and Ishikawa^shPiwil1 cells or in HEC-1B^EV and HEC-1B^exPiwil1 cells. Nuclei were stained with DAPI. Scale bars, 25 μm