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. 2015 Oct 27;10(10):e0141514. doi: 10.1371/journal.pone.0141514

Fig 4. Mutation analysis of SLC27A4 using complementary DNA.

Fig 4

(A) A loss of 54 bp is present in affected Great Danes homozygous for allele A (mut/mut) in the SNV SLC27A4:g.8684G>A. The PCR-product spans 601 bp from exon 7 to 11 in affected dogs and 655 bp in unaffected dogs homozygous for the wild type (wt/wt) whereas heterozygous dogs show both products (wt/mut). A very small amount of wild type PCR-product (655 bp) can also be seen in affected dogs. (B) PCR-product of affected and unaffected Great Danes using a primer placed into the first 54 bp of exon 8. It confirms that affected dogs also have small amount of RNA of wild type size.