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. 2015 Oct 27;12:24. doi: 10.1186/s12987-015-0021-y

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© Zuluaga-Ramirez et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Cannula components a from left to right, dummy, guide cannula and inner cannula. b Inner cannula inserted into guide cannula. c Cannula with screwed dummy. d Equipment for intracerebral (IC) injection. e Intracerebral injection in a fully anesthetize mouse, attached to stereotactic stage, using a nosepiece to deliver 2.0 % isoflurane and deliver insult via Hamilton syringe attached to an infusion cannula