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PGRN binds to TNFR on COOH1 chip, but not on COOHV chip in SensiQ surface plasmon resonance assay. (A) TNF binds to TNFR1 (red line) and TNFR2 (blue line) in SensiQ COOHV chip. (B) PGRN fails to bind to both TNFR1 (red line) and TNFR2 (blue line) on COOHV chip. (C) TNF-α binds to TNFR1α (red line) and TNFR2 (blue line) on COOH1 chip. (D) PGRN from adipogen binds to both TNFR1 (red line) and TNFR2 on COOH1 (blue line) chip. Adapted from Jian J, et al, FEBS letters, 2013
