Skip to main content
. 2015 Oct 27;10(10):e0141303. doi: 10.1371/journal.pone.0141303

Fig 3. Effect of R104P mutant on GPR40 mediated ERK phosphorylation.

Fig 3

HEK293 cells transiently transfected with wild type (A) or R104P mutant (B) or empty vector (C) were stimulated with different ligands at indicated concentration at 10min or 100 μM LA for various durations (0, 5, 10, 15, 30 and 60 min) at 37°C, and ERK1/2 phosphorylation was detected with Western blot. ERK phosphorylation levels were normalized to the GAPDH level in the same sample. Data are means ± SEM (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001, versus vehicle control.