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. 2015 Oct 27;10(10):e0141544. doi: 10.1371/journal.pone.0141544

Fig 3. X-linked transgenes are not fully dosage compensated in L. cuprina.

Fig 3

qRT-PCR of marker gene (ZsGreen or DsRed-express2) RNA in hemisected adult males and females from the transgenic lines SLAM5, EF 1–2 or DR3-9. All lines carry a single copy of the marker gene on the X chromosome. Transcript levels were normalized to 28S rRNA. As expression of the marker genes was driven by the constitutive promoter from the autosomal Lchsp83 gene, Lchsp83 transcript levels are shown for comparison. Mean female/male ratio +/- standard error from three biologically independent replicate experiments are shown.